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The local cytokine and growth factor response to recombinant human bone morphogenetic protein-2 (rhBMP-2) after spinal fusion.

by
The local cytokine and growth factor response to recombinant human bone morphogenetic protein-2 (rhBMP-2) after spinal fusion.

The systemic response relating to cytokine expression after the applying of recombinant human bone morphogenetic protein-2 (rhBMP-2) in a rat spinal fusion mannequin has lately been outlined, however the native response has not been outlined. Defining the native cytokine and development issue response on the fusion web site will assist clarify the roles of those molecules within the fusion course of, in addition to that of rhBMP-2. Our speculation is that the applying of rhBMP-2 to the fusion web site will alter the native ranges of cytokines and development elements all through the fusion course of, in a fashion that’s totally different from the systemic response, given the tissue-specific results of rhBMP-2. The aim of this examine was to guage the native cytokine and development issue response after the applying of rhBMP-2 in a rat spinal fusion mannequin.

This was a primary science animal mannequin examine. This examine was partially funded by a physician-sponsored grant from Medtronic. A complete of 135 Wistar rats (age eight weeks, weighing roughly 300-400 g) underwent L4-L5 posterolateral intertransverse fusion with demineralized bone graft (roughly 0.4-cm3 rat demineralized bone matrix [DBM] per aspect). Within the first group, 10 µg of rhBMP-2 on an allograft collagen sponge (ACS) was added to the fusion web site with roughly 0.4-cm3 rat DBM per aspect. Within the second group, 100 µg of rhBMP-2 on an ACS was added to the fusion web site with roughly 0.4-cm3 rat DBM per aspect, and the third experiment was the management group, which consisted of solely an ACS plus 0.4-cm3 DBM per aspect.

There have been 9 teams of 5 animals every per experiment. Every group was sacrificed at time factors as much as Four weeks (1, 6, 24, and 48 hours, and 4, 7, 14, 21, and 28 days after surgical procedure). At sacrifice, the DBM, transverse processes, and any new bone fashioned had been harvested, instantly frozen in liquid nitrogen, and ready for protein extraction. ELISA was carried out to match the degrees of assorted cytokines (interleukin [IL]-1β, tumor necrosis issue alpha, IL-6, IL-1RA [IL-1 receptor antagonist], IL-4, and IL-10) and development elements (vascular endothelial development issue [VEGF], endothelia development issue [EGF], insulin-like development factor-1 [IGF-1], platelet derived development issue [PDGF], remodeling development issue beta [TGF-β]) which might be identified to be concerned within the fusion-fracture therapeutic course of. Fusion was evaluated on the rats sacrificed at 28 days by guide palpation and microcomputed tomography (microCT) by two

The expression of cytokines and development elements various all through the fusion course of at every time level. Within the teams handled with rh-BMP-2, IL-6 and IL-1RA had greater expression within the early time factors (1 and 6 hours). Tumor necrosis issue alpha demonstrated considerably decrease expression within the teams handled with rhBMP-2 at Days 1, 2, and 4. On the early time factors (1 and 6 hours), within the teams handled with rhBMP-2, all the development elements IGF-1, VEGF, platelet derived development issue AB (PDGF-AB), TGF-β had equal or decrease expression in contrast with controls.

At 24 hours, there was a peak in IGF-1, VEGF, and PDGF-AB. These development elements then declined, with IGF-1 and PDGF-AB having a second peak at Day 7. At Four weeks, all the rhBMP-2-treated animals fused primarily based on guide palpation and microCT. The management group had 4 of 5 rats fused primarily based on guide palpation and two of 5 rats primarily based on microCT.

The local cytokine and growth factor response to recombinant human bone morphogenetic protein-2 (rhBMP-2) after spinal fusion.

Recombinant adenoviral expression of IL-10 protects beta cell from impairment induced by pro-inflammatory cytokine.

Interleukin-10 (IL-10) is a pleiotropic immunosuppressive and immunostimulatory cytokine. In autoimmune diabetes of the nonobese diabetic (NOD) mouse, IL-10 has exhibited paradoxical results. Systemic IL-10 expression prevented or delayed diabetes onset in NOD mice whereas native expression of IL-10 didn’t. As antigen-presenting cells (APCs) play a central function within the era of major T cell responses, the direct function of this gene in pancreatic beta (β) cell isn’t clear. The results of IL-10 on the safety of β cells in vitro had been examined. Within the current examine, we examined the results of adenovirus vector-mediated murine IL-10 (mIL-10) gene switch to islet cell line RINm5F cells in vitro and to discover if IL-10 overexpression could forestall cytokine-mediated cytotoxicity.

We had established the recombinant adenovirus vector containing mIL-10 genes (Advert-mIL-10) efficiently. After an infection of Advert-mIL-10, each mRNA and protein had been expressed in RINm5F cells. Furthermore, RINm5F cells secreted IL-10 protein into tradition medium. Advert-mIL-10 prevented IL-1β-mediated nitric oxide manufacturing from β cells in vitro in addition to the suppression of β cells operate as decided by glucose-stimulated insulin manufacturing. Moreover, Advert-mIL-10 gene switch led to a profound discount of Fas-expressing β cells and caspase-Three exercise which had been induced by IL-1β and the apoptotic charges of Advert-mIL-10 group had been decreased. These findings present that IL-10 gene switch to β cells could also be useful in sustaining cells operate, defending islet cells from apoptosis-mediated by elements, which confirmed the potential remedy for sort 1 diabetes mellitus.

Aligned Nanofibers 96 Well Plate
3D00012-96 700 nm-PCLs
EUR 110

96 WELL PCR SEGMENTED PLATE
PCR-96-SG-C 10/pk
EUR 145
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Well Breast Tumor Genomic DNA Plate
D8235086-1 1 plate
EUR 316

96 Well Colon Tumor Genomic DNA Plate
D8235090-1 1 plate
EUR 316

96 Well Lung Tumor Genomic DNA Plate
D8235152-1 1 plate
EUR 316

96 Well Stomach Tumor Genomic DNA Plate
D8235248-1 1 plate
EUR 316

Polystyrene-bottomed 96-well plate (ELISA)
CT361 6
EUR 73

Adapter for 96 well plate
3510147 1unit
EUR 5581

Black 96 Well Plate, 1EA
X025-1EA 1EA
EUR 73

Streptavidin coated 96-well Plate
6523-5
EUR 327

96 well filter plate (960ul each well)
SD5006 12UNIT
EUR 96.54

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
EXOEL-96P-1 10 Plates
EUR 213

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001 1 prep
EUR 146

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C 10/pk
EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Well Human Adult Normal Genomic DNA Plate - Peripheral Blood Leukocyte
D8234148-1 1 plate
EUR 256

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY
P-DW-12-HC 5/pk
EUR 152
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

96-Well Plate PCR Products Purification Kit
BS365 5XPlates, 480prep
EUR 250.31

96-Well Plate PCR Products Purification Kit
BS3652 2xPlates, 192prep
EUR 155.16

96-Well Plate DNA Cleanup Miniprep Kit
BS369 5XPlates, 480prep
EUR 250.31

96-Well Plate DNA Cleanup Miniprep Kit
BS3692 2xPlates, 192prep
EUR 155.16

96-Well Plate Plasmid DNA Miniprep Kit
BS415 5XPlates, 480prep
EUR 250.31

96-Well Plate Plasmid DNA Miniprep Kit
BS4152 2XPlates, 192prep
EUR 155.16

96-Well Plate Viral DNA Miniprep Kit
VT92032 2XPlates, 192prep
EUR 413.22

96 well filter plate (no filter)
SD5006-EMPTY 12UNIT
EUR 87.41

Clear Coated 96 Well Plate, 1EA
C107-1EA 1EA
EUR 189

OxiSelect 96-Well Comet Assay Kit
STA-355 96 assays
EUR 427
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit
STA-355-5 5 x 96 assays
EUR 1633
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

96-Well ROCK Activity Assay Kit
STA-416 96 assays
EUR 856
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit
STA-416-5 5 x 96 assays
EUR 3524
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE
PCR-96-HS-C 10/pk
EUR 159
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.
CP-AXYGEM-96-50 10/pk
EUR 457
Description: Crystallography Plates; Crystallography Plates - Axygen

96-well Plasmid ezFilter Mini Kit
K1325-96-1
EUR 370

96 well DNA plate with membrane (960ul each well)
SD5007 12UNIT
EUR 242.7

96 well RNA plate with membrane (960ul each well)
SD5009 12UNIT
EUR 260.97

96 Well Plate RNA Cleanup and Concentration Kit
BRC1251 2xPlates, 192prep
EUR 250.31

96-Well Plate Animal Genomic DNA Miniprep Kit
BS437 5XPlates, 480prep
EUR 362.98

96-Well Plate Animal Genomic DNA Miniprep Kit
BS4372 2XPlates, 192prep
EUR 195.5

96 Well Plate Yeast Genomic DNA Miniprep Kit
BS8357 2XPlates, 192prep
EUR 195.5

96-Well Plate Plant Genomic DNA Miniprep Kit
BS8361 2XPlates, 192prep
EUR 195.5

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031 2Plates, 192prep
EUR 175.71

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032 5Plates, 480prep
EUR 324.92

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1292 2XPlates, 192prep
EUR 175.71

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1295 5XPlates, 480prep
EUR 324.92

Polystyrene-bottomed 96-well plate + lid (ELISPOT)
CT365 5
EUR 78

2 x Clear 96 well Plate, 2EA
X003-2EA 2EA
EUR 65

5 x Clear 96 well Plate, 5EA
X003-5EA 5EA
EUR 80

2 x Clear 96 well Plate, 2EA
X018-2EA 2EA
EUR 65

4 x Clear 96 Well Plate, 4EA
X018-4EA 4EA
EUR 87

Black Half Area 96 Well Plate, 1EA
X023-1EA 1EA
EUR 80

96-well Checkpoint Kinase Activity Assay Kit
STA-414 96 assays
EUR 757
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit
STA-414-5 5 x 96 assays
EUR 3060
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit
25-224 100 plates
EUR 299
Description:

Suitable for cell culture but only for suspension cells

  • Optically clear with low autofluorescence for distortion free microscopy
  • Low evaporation lid reduces evaporation and prevents contamination
  • Exterior stacking ring on lid for added stability when stacking
  • Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
  • Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

Radius 96-Well Cell Migration Assay
CBA-126 96 assays
EUR 572
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay
CBA-126-5 5 x 96 assays
EUR 2248
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay
CBA-130 96 assays
EUR 722
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay
CBA-130-5 5 x 96 assays
EUR 2886
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224 96 assays
EUR 722
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224-5 5 x 96 assays
EUR 2938
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

OxiSelect 96-Well Comet Assay Slide
STA-356 1 slide
EUR 183
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide
STA-356-5 5 slides
EUR 519
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED
P-DW-12-HC-S 5/pk
EUR 207
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

96-Well Rack, Reversible, Blue
R577-B 1 UNIT
EUR 54.05

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)
ACT-SL-96
EUR 647

96-well Viral DNA/RNA Kit
K1417-1
EUR 392

6 WELL BLUE PCR FULL SKIRT AMPLIFICATION PLATE.
PCR-96-FS-B 10/pk
EUR 176
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS585 5XPlates, 480prep
EUR 487.82

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS5852 2XPlates, 192prep
EUR 250.31

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92028 2XPlates, 192prep
EUR 212.25

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92029 5XPlates, 480prep
EUR 364.5

PCR Master Mix, Ready To Load (96 well, 12x8 strip-well plate, lyophilized without loading dye)
MB1001-1-1plate96well12x8strips 1 plate (96 well, 12x8 strips)
EUR 143

PCR Master Mix, Ready To Load (96 well, 12x8 strip-well plate, lyophilized without loading dye)
MB1001-1-5plates96well12x8strips 5 plates (96 well, 12x8 strips)
EUR 375

Block for 96 well plate, WSC-2620/30
4002659 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
CT366 5
EUR 224

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
ELISA-1 1
EUR 202

96-Well PCR Tube Racks, Blue
R541-B 1 UNIT
EUR 53.05

Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA
X011-1EA 1EA
EUR 102

soluble collagen assay kit 96-assays
QZBcol1-1 1 plate 96 assays
EUR 334

DNA-BIND™ PLATE, 96 WELL, CLEAR, FLAT 1/10
2525 1/pk
EUR 137
Description: Genomics; Genomic Plates

Mouse anti-Endothelin-1 Clear Coated 96 Well Plate, 1EA
C161-1EA 1EA
EUR 178

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)
CBA-081 96 assays
EUR 606
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)
B814152-0002 2XPlates, 2XPlates
EUR 155.16

96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based)
B814152-0005 5XPlates, 5XPlates
EUR 250.31

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTA55-5P 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTG65-5P 1 PK
EUR 263

Goat anti-Mouse, 1 x 8 Strip Well, Clear Coated 96 Well Plate, 1EA
X012-1EA 1EA
EUR 109

Adaptor for 96-well PCR plate, accessory for AgileSealer?
APS-200-96PCR
EUR 351

Block for 96 deep-well plate, WSC-2620/30
4002661 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE
432071 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480130 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES
480131 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480132 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

96 Well PCR Plates, Fits ABI Fast Plate Machines
GP615 25 plates
EUR 212

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA
C075-1EA 1EA
EUR 178

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA
C140-1EA 1EA
EUR 178

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA
C223-1EA 1EA
EUR 178

Goat anti-Mouse, Coated White 96 Well Plate, 1EA
X010-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA
X014-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA
X016-1EA 1EA
EUR 109

A 66-year-old affected person with myelopathy underwent posterior cervical decompression and fusion, utilizing rhBMP-2 as a bone graft substitute. The affected person had full decision of signs after surgical procedure till day 6, when she skilled rising ache and weak spot. T2 magnetic resonance photographs revealed a excessive depth fluid assortment compressing the cervical wire posteriorly. Emergent decompression was carried out and the affected person improved till postoperative day 12 when the identical medical state of affairs occurred. Signs once more improved with surgical debridement. The clear, nonsanguineous fluid was despatched for a quantitative cytokine panel every time. The case is reviewed with particular reference to the evolving literature relating to rhBMP-2 use within the backbone, and the findings of seroma evaluation.

Recombinant human proteoglycan-4 reduces phagocytosis of urate crystals and downstream nuclear factor kappa B and inflammasome activation and production of cytokines and chemokines in human and murine macrophages.

by
Recombinant human proteoglycan-4 reduces phagocytosis of urate crystals and downstream nuclear factor kappa B and inflammasome activation and production of cytokines and chemokines in human and murine macrophages.

Gout is an inflammatory arthritis brought on by monosodium urate monohydrate (MSU) crystals’ joint deposition. MSU phagocytosis by resident macrophages is a key step in gout pathogenesis. MSU phagocytosis triggers nuclear issue kappa B (NFκB) activation and manufacturing of cytokines and chemokines. Proteoglycan-4 (PRG4) is a glycoprotein produced by synovial fibroblasts and exerts an anti-inflammatory impact within the joint mediated by its interplay with cell floor receptor CD44. PRG4 additionally binds and antagonizes TLR2 and TLR4. The target of this research is to guage the efficacy of recombinant human PRG4 (rhPRG4) in suppressing MSU-induced irritation and mechanical allodynia in vitro and in vivo.

THP-1 macrophages have been incubated with MSU crystals ± rhPRG4 or bovine submaxillary mucin (BSM), and crystal phagocytosis, cytokines and chemokines expression and manufacturing have been decided. NFκB p65 subunit nuclear translocation, NLRP3 induction, caspase-1 activation and conversion of proIL-1β to mature IL-1β have been studied. MSU phagocytosis by Prg4+/+ and Prg4-/- peritoneal macrophages was decided within the absence or presence of rhPRG4, BSM, anti-CD44, anti-TLR2, anti-TLR4 and isotype management antibodies. Rhodamine-labeled rhPRG4 was incubated with murine macrophages and receptor colocalization research have been carried out. Lewis rats underwent intra-articular injection of MSU crystals adopted by intra-articular therapy with PBS or rhPRG4. Weight bearing and SF myeloperoxidase actions have been decided.

rhPRG4 decreased MSU crystal phagocytosis at Four h (p < 0.01) and IL-1β, TNF-α, IL-Eight and MCP-1 expression and manufacturing at 6 h (p < 0.05). BSM didn’t alter MSU phagocytosis or IL-1β manufacturing in human and murine macrophages. rhPRG4 therapy decreased NFκB nuclear translocation, NLRP3 expression, caspase-1 activation and era of mature IL-1β (p < 0.05). MSU-stimulated IL-1β manufacturing was greater in Prg4-/- macrophages in comparison with Prg4+/+ macrophages (p < 0.001). rhPRG4, anti-CD44, anti-TLR2 and anti-TLR4 antibody therapies decreased MSU phagocytosis and IL-1β manufacturing in murine macrophages (p < 0.05). rhPRG4 preferentially colocalized with CD44 on Prg4-/- peritoneal macrophages in comparison with TLR2 or TLR4 (p < 0.01). rhPRG4 normalized weight bearing and decreased SF myeloperoxidase exercise in comparison with PBS in vivo. rhPRG4 inhibits MSU crystal phagocytosis and reveals an anti-inflammatory and anti-nociceptive exercise in vitro and in vivo. rhPRG4’s anti-inflammatory mechanism could also be as a result of focusing on CD44 on macrophages.

Recombinant thrombomodulin ameliorates experimental autoimmune encephalomyelitis by suppressing excessive mobility group field 1 and inflammatory cytokines.

Recombinant thrombomodulin (rTM) has pleiotrophic properties, together with anti-coagulation and anti-inflammation; nonetheless, its effectiveness as a therapy for a number of sclerosis (MS) has not been evaluated totally. Excessive mobility group field 1 (HMGB1) and proinflammatory cytokines, working as inflammatory mediators, are reportedly concerned within the inflammatory pathogenesis of MS. The purpose of this research was to find out whether or not rTM generally is a potential therapeutic agent for experimental autoimmune encephalomyelitis (EAE). EAE mice obtained rTM therapy (1 mg or 0·1 mg/kg/day) from days 11 to 15 after immunization.

The scientific variables, plasma ranges of inflammatory cytokines and HMGB1 and pathological findings in EAE have been evaluated. rTM administration ameliorated the scientific and pathological severity of EAE. An immunohistochemical research of the spinal wire confirmed weaker cytoplasmic HMGB1 staining within the rTM-treated EAE mice than within the untreated EAE mice. Plasma ranges of inflammatory cytokines and HMGB1 have been suppressed by rTM therapy. In conclusion, rTM down-regulated inflammatory mediators within the peripheral circulation and prevented HMGB1 launch from nuclei within the central nervous system, suppressing EAE-related irritation. rTM may have a novel therapeutic potential for sufferers with MS.

Recombinant human proteoglycan-4 reduces phagocytosis of urate crystals and downstream nuclear factor kappa B and inflammasome activation and production of cytokines and chemokines in human and murine macrophages.

IL-35 recombinant protein reverses inflammatory bowel illness and psoriasis by means of regulation of inflammatory cytokines and immune cells.

Interleukin-35 (IL-35), a member of the IL-12 household, features as a brand new anti-inflammatory issue concerned in arthritis, psoriasis, inflammatory bowel illness (IBD) and different immune ailments. Though IL-35 can considerably stop the event of irritation in lots of ailments, there have been no early research accounting for the function of IL-35 recombinant protein in IBD and psoriasis. On this research, we assessed the therapeutic potential of IL-35 recombinant protein in three well-known mouse fashions: the dextransulfate sodium (DSS)-induced colitis mouse mannequin, the keratin14 (Okay14)-vascular endothelial development issue A (VEGF-A)-transgenic (Tg) psoriasis mouse mannequin and the imiquimod (IMQ)-induced psoriasis mouse mannequin. Our outcomes indicated that IL-35 recombinant protein can decelerate the pathologic course of in DSS-induced acute colitis mouse mannequin by lowering the infiltrations of macrophages, CD4+ T and CD8+ T cells and by selling the infiltration of Treg cells.

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)
30011-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Acetylcholinesterase Activity Assay Kit - 100 Assays
AR4001-unit unit
EUR 317

Phosphate Assay Kit (Fluorometric)
K2076-100 100 assays
EUR 363

Glutathione Fluorometric Assay Kit
K2098-100 100 assays
EUR 502

Lactate Fluorometric Assay Kit
K2140-100 100 assays
EUR 641

Glucose Fluorometric Assay Kit
K2221-100 100 assays
EUR 557

Citrulline Fluorometric Assay Kit
K2002-100
EUR 620

Lysine Assay Kit (Fluorometric)
K2005-100
EUR 675

Glutathione Fluorometric Assay Kit
K251-100
EUR 479

Adenosine Assay Kit (Fluorometric)
K327-100
EUR 805

Arginine Assay Kit (Fluorometric)
K384-100
EUR 566

Histamine Assay Kit (Fluorometric)
K386-100
EUR 533

Calcium Assay Kit (Fluorometric)
K409-100
EUR 479

Phosphate Assay Kit (Fluorometric)
K420-100
EUR 349

Zinc Assay Kit (Fluorometric)
K428-100
EUR 523

Methionine Assay Kit (Fluorometric)
K442-100
EUR 664

Phosphatidylglycerol Assay Kit (Fluorometric)
K488-100
EUR 566

Phosphatidylethanolamine Assay Kit (Fluorometric)
K499-100
EUR 631

Homocysteine Assay Kit (Fluorometric)
K531-100
EUR 648

Tryptophan Assay Kit (Fluorometric)
K557-100
EUR 642

Cysteine Assay Kit (Fluorometric)
K558-100
EUR 561

Phosphatidylserine Assay Kit (Fluorometric)
K565-100
EUR 620

Phenylalanine Fluorometric Assay Kit
K572-100
EUR 479

Glycine Assay Kit (Fluorometric)
K589-100
EUR 691

Inosine Fluorometric Assay Kit
K712-100
EUR 533

Ornithine Assay Kit (Fluorometric)
K939-100
EUR 588

Cardiolipin Assay Kit (Fluorometric)
K944-100
EUR 729

Acetyl-CoA Fluorometric Assay Kit
K2028-100 100 assays
EUR 544

Calpain Activity Fluorometric Assay Kit
K2062-100 100 assays
EUR 627

Aspartate Colorimetric/Fluorometric Assay Kit
K2082-100 100 assays
EUR 502

Phosphatidylcholine Colorimetric/Fluorometric Assay Kit
K2086-100 100 assays
EUR 502

PLTP Activity Fluorometric Assay Kit
K2087-100 100 assays
EUR 669

CETP Activity Fluorometric Assay Kit
K2089-100 100 assays
EUR 725

Glucose Colorimetric/Fluorometric Assay Kit
K2091-100 100 assays
EUR 502

Lactate Colorimetric/Fluorometric Assay Kit
K2092-100 100 assays
EUR 614

Proteasome Activity Fluorometric Assay Kit
K2096-100 100 assays
EUR 502

GST Fluorometric Activity Assay Kit
K2105-100 100 assays
EUR 502

Pyruvate Colorimetric/Fluorometric Assay Kit
K2119-100 100 assays
EUR 614

Adipogenesis Colorimetric/Fluorometric Assay Kit
K2120-100 100 assays
EUR 447

Fructose Colorimetric/Fluorometric Assay Kit
K2124-100 100 assays
EUR 502

Ethanol Colorimetric/Fluorometric Assay Kit
K2125-100 100 assays
EUR 586

Galactose Colorimetric/Fluorometric Assay Kit
K2126-100 100 assays
EUR 502

Lactose Colorimetric/Fluorometric Assay Kit
K2129-100 100 assays
EUR 502

Creatinine Colorimetric/Fluorometric Assay Kit
K2130-100 100 assays
EUR 447

Maltose Colorimetric/Fluorometric Assay Kit
K2132-100 100 assays
EUR 502

Creatine Colorimetric/Fluorometric Assay Kit
K2137-100 100 assays
EUR 502

Sarcosine Colorimetric/Fluorometric Assay Kit
K2138-100 100 assays
EUR 502

Glycogen Colorimetric/Fluorometric Assay Kit
K2143-100 100 assays
EUR 614

Caspase-12 Fluorometric Assay Kit
K2150-100 100 assays
EUR 529

DPP4 Activity Fluorometric Assay Kit
K2178-100 100 assays
EUR 557

Caspase-5 Fluorometric Assay Kit
K2195-100 100 assays
EUR 502

Caspase-4 Fluorometric Assay Kit
K2198-100 100 assays
EUR 529

Alanine Colorimetric/Fluorometric Assay Kit
K2205-100 100 assays
EUR 502

Citrate Colorimetric/Fluorometric Assay Kit
K2207-100 100 assays
EUR 544

Glucose Uptake Fluorometric Assay Kit
K2212-100 100 assays
EUR 1198

Neuraminidase Activity Fluorometric Assay Kit
K2230-100 100 assays
EUR 599

Caspase-3 Fluorometric Assay Kit
K105-100
EUR 479

Caspase-1 Fluorometric Assay Kit
K110-100
EUR 479

Caspase-8 Fluorometric Assay Kit
K112-100
EUR 479

Caspase-6 Fluorometric Assay Kit
K114-100
EUR 430

Caspase-2 Fluorometric Assay Kit
K116-100
EUR 463

Caspase-9 Fluorometric Assay Kit
K118-100
EUR 468

Caspase-5 Fluorometric Assay Kit
K122-100
EUR 436

Caspase-10 Fluorometric Assay Kit
K124-100
EUR 441

Caspase-4 Fluorometric Assay Kit
K126-100
EUR 468

Caspase-12 Fluorometric Assay Kit
K139-100
EUR 501

Glucosylceramidase Activity Assay Kit (Fluorometric)
K2003-100
EUR 588

Transketolase Activity Assay Kit (Fluorometric)
K2004-100
EUR 620

Carboxylesterase Activity Assay Kit (Fluorometric)
K2014-100
EUR 620

Inorganic Polyphosphate Assay Kit (Fluorometric)
K2025-100 100 assays
EUR 551

CD38 Activity Assay Kit (Fluorometric)
K2042-100 100 assays
EUR 581

N-Acetylcysteine Assay Kit (Fluorometric)
K2044-100 100 assays
EUR 581
Description:

Simple, Sensitive method for the quantitation of NAC in biological samples, The assay is specific with no interference from other thiol-based amino acids.

N-Acetylcysteine (NAC) is a precursor of L-cysteine and glutathione biosynthesis. It is a powerful antioxidant and a scavenger of free radicals. It has been commonly used for treating cough, flu, dry eye, lung conditions, paracetamol intoxication etc. Additionally, recent investigations have explored the beneficial effects of NAC in type 2 diabetes and psychiatric disorders. NAC is available as over the counter in tablet, solution as well as intravenous and inhaled preparations. Common side effects of NAC treatment are generally mild and typically resolve on their own once the treatment is stopped. BioVision’s N-Acetylcysteine Assay Kit is a simple and sensitive method for the quantitation of NAC in biological samples. The assay is based on the deacetylation reaction of NAC to generate cysteine. Cysteine is then catalyzed in subsequent reactions thereby producing an intermediate product, which reacts with a fluorogenic probe to form a stable fluorophore measured at Ex/Em = 368/460 nm. The assay is specific with no interference from other thiol-based amino acids.

Sialyltransferase Activity Assay Kit (Fluorometric)
K2048-100 100 assays
EUR 601

PicoProbe? ADP Assay Kit (Fluorometric)
K211-100
EUR 582

Lysozyme Activity Assay Kit (Fluorometric)
K236-100
EUR 490

Calpain Activity Fluorometric Assay Kit
K240-100
EUR 588

Proteasome Activity Fluorometric Assay Kit
K245-100
EUR 479

GST Fluorometric Activity Assay Kit
K260-100
EUR 468

Sirtuin Activity Assay Kit (Fluorometric)
K324-100
EUR 572

HDAC Activity Fluorometric Assay Kit
K330-100
EUR 490

HAT Activity Fluorometric Assay Kit
K334-100
EUR 539

HDAC3 Activity Fluorometric Assay Kit
K343-100
EUR 479

HDAC8 Activity Fluorometric Assay Kit
K348-100
EUR 490

Phospholipid Assay Kit (Colorimetric/Fluorometric)
K351-100
EUR 550

Chymotrypsin Activity Assay Kit (Fluorometric)
K352-100
EUR 490

ATP Colorimetric/Fluorometric Assay Kit
K354-100
EUR 582

ADP Colorimetric/Fluorometric Assay Kit
K355-100
EUR 582

FAD Colorimetric/Fluorometric Assay Kit
K357-100
EUR 512

PEP Colorimetric/Fluorometric Assay Kit
K365-100
EUR 631

Thrombin Activity Fluorometric Assay Kit
K373-100
EUR 697

Plasmin Activity Assay Kit (Fluorometric)
K381-100
EUR 457

PicoProbe? Glutamate Assay Kit (Fluorometric)
K413-100
EUR 566

PicoProbe? Phosphate Fluorometric Assay Kit
K419-100
EUR 446

Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric)
K433-100
EUR 566

PicoProbeTM Methylglyoxal Assay Kit (Fluorometric)
K461-100
EUR 555

PicoProbe? Threonine Assay Kit (Fluorometric)
K463-100
EUR 610

Additional evaluation demonstrated that IL-35 recombinant protein might regulate irritation by means of selling the secretion of IL-10 and inhibiting the expression of pro-inflammatory cytokines similar to IL-6, TNF-α and IL-17 in acute colitis mannequin. As well as, decrease dose of IL-35 recombinant protein may obtain long-term therapy results as TNF-α monoclonal antibody did within the psoriasis mouse. In abstract, the outstanding therapeutic results of IL-35 recombinant protein in acute colitis and psoriasis mouse fashions indicated that IL-35 recombinant protein had a wide range of anti-inflammatory results and was anticipated to develop into an efficient candidate drug for the therapy of inflammatory ailments. Elevated data of the immune response of the gut, a physiologically crucial organ concerned in absorption, secretion and homeostasis in a non-sterile atmosphere, is required to raised perceive the mechanisms concerned within the induction of long-lasting immunity and, subsequently, the event of efficacious gastrointestinal immunization approaches. To this finish, evaluation of remoted intestine cells will give an perception into the cell varieties current and their immune functionality. Therefore, on this research we first optimised a way for salmonid intestine leucocyte isolation and characterised the cells on the premise of their expression of a variety of chosen cell markers related to T & B cells and dendritic cells.

SOCS4 expressed by recombinant HSV protects against cytokine storm in a mouse model.

by
SOCS4 expressed by recombinant HSV protects against cytokine storm in a mouse model.

Oncolytic viruses are genetically engineered viruses designed for the remedy of stable tumors, and are sometimes coupled with the antitumor immunity of the host. The problem of utilizing oncolytic herpes simplex virus (oHSV) as an efficacious oncolytic agent is the potential host tissue harm attributable to the manufacturing of a variety of cytokines following intratumoral oHSV injection. An HSV‑suppressor of cytokine signaling 4 (SOCS4) recombinant virus was created to analyze whether or not it inhibits cytokine storm.

Recombinant HSV‑SOCS4 and HSV‑1(F) have been used to contaminate mice, and ranges of a number of consultant cytokines, together with monocyte chemoattractant protein‑1, interleukin (IL)‑1β, tumor necrosis issue‑α, IL‑6 and interferon γ, in serum and bronchoalveolar lavage fluid (BALF) of contaminated mice have been decided, and immune cells in BALF and spleen have been enumerated. Lung harm, virus titers within the lung, physique weight and survival charges of contaminated mice have been additionally decided and in contrast between the 2 teams.

The cytokine focus of HSV‑SOCS4‑contaminated mice was considerably decreased in contrast with that of HSV‑1(F)‑contaminated mice in BALF and serum, and a smaller variety of cluster of differentiation (CD)11b+ cells of BALF, and CD8+CD62L+ T cells and CD4+CD62L+ T cells of the spleen have been additionally recognized in HSV‑SOCS4‑contaminated mice. HSV‑SOCS4‑contaminated mice exhibited slight lung harm, a lower in physique weight reduction and a 100% survival price.

The outcomes of the current examine indicated that SOCS4 protein could also be a helpful regulator to inhibit cytokine overproduction, and that HSV‑SOCS4 could present a doable resolution to regulate cytokine storm and its penalties following induction by oncolytic virus remedy. On this examine, the antitumor exercise of the recombinant adenovirus KGHV500 was assessed with the MTT, TUNEL, Matrigel invasion and cell migration assays. To boost the intravenous supply of KGHV500 in vivo, cytokine-induced killer (CIK) cells have been used as a second vector to hold KGHV500. We explored whether or not CIK cells may carry the recombinant adenovirus KGHV500 containing the anti-p21Ras single chain fragment variable antibody (scFv) gene into tumors and improve antitumor efficiency.

A canine keratinocyte cell line expresses antimicrobial peptide and cytokine genes upon stimulation with micro organism, microbial ligands and recombinant cytokines.

Keratinocytes (KC) are the primary mobile elements of the stratum corneum that constitutes a stable bodily pores and skin barrier representing the primary line of protection towards pathogens. Furthermore, KC are potent producers of inflammatory mediators and antimicrobial peptides (AMP) when activated by way of their sample recognition receptors. In atopic dermatitis (AD) the protecting pores and skin barrier could also be compromised attributable to barrier disruption, secondary an infection and accelerated secretion of inflammatory cytokines which can additionally have an effect on AMP expression within the pores and skin. Within the current examine, we addressed the responses of a canine KC cell line upon publicity to Staphylococcus pseudintermedius, usually discovered on canine atopic pores and skin throughout secondary infections, and stimulation by particular person AD-associated ligands and cytokines.

All stimuli induced a major improve in expression of the pro-inflammatory cytokine genes tumor necrosis issue (TNF)-α and interleukin (IL)-8, however with totally different kinetics. Restricted results have been noticed on AMP gene expression aside from K9CATH which was considerably upregulated upon bacterial an infection however with not one of the particular person AD-associated ligands. Apparently, K9CATH possessed antimicrobial exercise in the direction of Staphylococcus pseudintermedius, indicating that K9CATH expression is a selected protection response in the direction of bacterial an infection and never a part of a basic pro-inflammatory profile of KC.

SOCS4 expressed by recombinant HSV protects against cytokine storm in a mouse model.

The Results of Mouse Recombinant Resistin on mRNA Expression of Proinflammatory and Anti-Inflammatory Cytokines and Warmth Shock Protein-70 in Experimental Stroke Mannequin.

Our current analysis confirmed that resistin has a neuroprotective impact towards stroke-induced damage by way of suppressing apoptosis and oxidative stress. Nevertheless, the molecular mechanism of neuroprotection of resistin is unclear. This work was designed to look at the impact of mouse recombinant resistin on mRNA expression of Tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Interleukin-10 (IL-10), Reworking progress factor-β1 (TGF- β1), and Warmth shock protein-70 (HSP-70) in mouse mannequin of stroke.

Cytokines regulate nearly elements of innate and adaptive immunity, together with the initiation, execution and extinction of tumor-targeting immune responses. Over the previous three a long time, the opportunity of utilizing recombinant cytokines as a method to elicit or increase clinically related anticancer immune responses has attracted appreciable consideration. Nevertheless, solely three cytokines have been authorised up to now by the US Meals and Drug Administration and the European Medicines Company to be used in most cancers sufferers, specifically, recombinant interleukin (IL)-2 and two variants of recombinant interferon alpha 2 (IFN-α2a and IFN-α2b).

Adapter for 96 well plate
3510147 1unit
EUR 5581

Streptavidin coated 96-well Plate
6523-5
EUR 327

96 well filter plate (960ul each well)
SD5006 12UNIT
EUR 96.54

96 WELL CLEAR PCR PLATE FOR ABI
PCR-96-AB-C 10/pk
EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY
P-DW-12-HC 5/pk
EUR 152
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Clear Coated 96 Well Plate, 1EA
C107-1EA 1EA
EUR 189

96 well filter plate (no filter)
SD5006-EMPTY 12UNIT
EUR 87.41

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE
PCR-96-HS-C 10/pk
EUR 159
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.
CP-AXYGEM-96-50 10/pk
EUR 457
Description: Crystallography Plates; Crystallography Plates - Axygen

96 well DNA plate with membrane (960ul each well)
SD5007 12UNIT
EUR 242.7

96 well RNA plate with membrane (960ul each well)
SD5009 12UNIT
EUR 260.97

2 x Clear 96 well Plate, 2EA
X003-2EA 2EA
EUR 65

5 x Clear 96 well Plate, 5EA
X003-5EA 5EA
EUR 80

2 x Clear 96 well Plate, 2EA
X018-2EA 2EA
EUR 65

4 x Clear 96 Well Plate, 4EA
X018-4EA 4EA
EUR 87

Black Half Area 96 Well Plate, 1EA
X023-1EA 1EA
EUR 80

96-Well Plate Viral DNA Miniprep Kit
VT92032 2XPlates, 192prep
EUR 413.22

96-Well Plate PCR Products Purification Kit
BS365 5XPlates, 480prep
EUR 250.31

96-Well Plate PCR Products Purification Kit
BS3652 2xPlates, 192prep
EUR 155.16

96-Well Plate DNA Cleanup Miniprep Kit
BS369 5XPlates, 480prep
EUR 250.31

96-Well Plate DNA Cleanup Miniprep Kit
BS3692 2xPlates, 192prep
EUR 155.16

96-Well Plate Plasmid DNA Miniprep Kit
BS415 5XPlates, 480prep
EUR 250.31

96-Well Plate Plasmid DNA Miniprep Kit
BS4152 2XPlates, 192prep
EUR 155.16

Polystyrene-bottomed 96-well plate + lid (ELISPOT)
CT365 5
EUR 78

96 Well Breast Tumor Genomic DNA Plate
D8235086-1 1 plate
EUR 316

96 Well Colon Tumor Genomic DNA Plate
D8235090-1 1 plate
EUR 316

96 Well Lung Tumor Genomic DNA Plate
D8235152-1 1 plate
EUR 316

96 Well Stomach Tumor Genomic DNA Plate
D8235248-1 1 plate
EUR 316

OxiSelect 96-Well Comet Assay Kit
STA-355 96 assays
EUR 427
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit
STA-355-5 5 x 96 assays
EUR 1633
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Slide
STA-356 1 slide
EUR 183
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide
STA-356-5 5 slides
EUR 519
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96-Well ROCK Activity Assay Kit
STA-416 96 assays
EUR 856
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit
STA-416-5 5 x 96 assays
EUR 3524
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

Radius 96-Well Cell Migration Assay
CBA-126 96 assays
EUR 572
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay
CBA-126-5 5 x 96 assays
EUR 2248
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay
CBA-130 96 assays
EUR 722
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay
CBA-130-5 5 x 96 assays
EUR 2886
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224 96 assays
EUR 722
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)
CBA-224-5 5 x 96 assays
EUR 2938
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED
P-DW-12-HC-S 5/pk
EUR 207
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)
ACT-SL-96
EUR 647

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1292 2XPlates, 192prep
EUR 175.71

96-Well Plate Bacteria Genomic DNA Miniprep Kit
SK1295 5XPlates, 480prep
EUR 324.92

96-well Plasmid DNA Extraction Kit (1 plate)
FAPWE-96001 1 prep
EUR 146

96 Well Plate RNA Cleanup and Concentration Kit
BRC1251 2xPlates, 192prep
EUR 250.31

96-Well Plate Animal Genomic DNA Miniprep Kit
BS437 5XPlates, 480prep
EUR 362.98

96-Well Plate Animal Genomic DNA Miniprep Kit
BS4372 2XPlates, 192prep
EUR 195.5

96 Well Plate Yeast Genomic DNA Miniprep Kit
BS8357 2XPlates, 192prep
EUR 195.5

96-Well Plate Plant Genomic DNA Miniprep Kit
BS8361 2XPlates, 192prep
EUR 195.5

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92031 2Plates, 192prep
EUR 175.71

96 Well Plate Blood Genomic DNA Miniprep Kit
BT92032 5Plates, 480prep
EUR 324.92

Block for 96 well plate, WSC-2620/30
4002659 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
CT366 5
EUR 224

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)
EXOEL-96P-1 10 Plates
EUR 213

96-well Checkpoint Kinase Activity Assay Kit
STA-414 96 assays
EUR 757
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit
STA-414-5 5 x 96 assays
EUR 3060
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)
CBA-081 96 assays
EUR 606
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit
25-224 100 plates
EUR 299
Description:

Suitable for cell culture but only for suspension cells

  • Optically clear with low autofluorescence for distortion free microscopy
  • Low evaporation lid reduces evaporation and prevents contamination
  • Exterior stacking ring on lid for added stability when stacking
  • Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
  • Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTA55-5P 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTG65-5P 1 PK
EUR 263

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA
C075-1EA 1EA
EUR 178

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA
C140-1EA 1EA
EUR 178

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA
C223-1EA 1EA
EUR 178

Goat anti-Mouse, Coated White 96 Well Plate, 1EA
X010-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA
X014-1EA 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA
X016-1EA 1EA
EUR 109

2 x Black Half Area 96 Well Plate, 2EA
X037-2EA 2EA
EUR 94

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA
X061-1EA 1EA
EUR 109

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA
X063-1EA 1EA
EUR 109

96 Well PCR Plates, Fits ABI Fast Plate Machines
GP615 25 plates
EUR 212

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE
432071 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480130 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES
480131 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED
480132 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS585 5XPlates, 480prep
EUR 487.82

96 Well Plate Bacterial Total RNA Miniprep Super Kit
BS5852 2XPlates, 192prep
EUR 250.31

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92028 2XPlates, 192prep
EUR 212.25

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit
BS92029 5XPlates, 480prep
EUR 364.5

Adaptor for 96-well PCR plate, accessory for AgileSealer?
APS-200-96PCR
EUR 351

Block for 96 deep-well plate, WSC-2620/30
4002661 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML
PCR-96-AB-C-PBC 10/pk
EUR 195
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)
CBA-320 96 assays
EUR 537
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well
1018-96 1/EA
EUR 518

Clear 384 Well Plate
X105-1EA 1EA
EUR 112

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric
CBA-112-COL 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric
CBA-112-LN 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)
CBA-220 96 assays
EUR 635
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-well Cellular Senescence Assay (SA ?-Gal Activity)
CBA-231 120 assays
EUR 635
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

96-well Cellular Senescence Assay (SA ?-Gal Activity)
CBA-231-5 5 x 120 assays
EUR 2648
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack
PRTAG75-5P 1 PK
EUR 286

Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA
X011-1EA 1EA
EUR 102

Mouse anti-Cystatin C Clear Coated 96 Well Plate, 1EA
C033-1EA 1EA
EUR 178

Mouse anti-Endothelin-1 Clear Coated 96 Well Plate, 1EA
C161-1EA 1EA
EUR 178

Transient focal cerebral ischemia was induced by the center cerebral artery occlusion (MCAO) in mice. TNF-α, IL-1β, IL-10, TGF-β1, and HSP-70 mRNA have been detected at sham (zero hour), three hours, 6 hours, 12 hours, and 24 hours after MCAO utilizing real-time QRT-PCR methodology. Furthermore, animals have been handled with resistin on the dose of 400ng/mouse on the graduation of MCAO, and mRNA expression of the cytokines and HSP-70 was measured 24 hours after MCAO. Tumor necrosis factor-α and IL-1β mRNA expression markedly elevated at 12-hour time level after which returned to the basal degree at 24 hours after MCAO; however HSP-70 mRNA expression elevated at 24-hour time level. Moreover, resistin (400 ng/mouse) considerably elevated TGF-β1 and IL-10 and decreased HSP-70 gene expression at 24 hours after MCAO.

Recombinant C-terminal heparin-binding domain of fibronectin polypeptide protects against liver damage, reduces serum inflammatory cytokines, and decreases mortality in acute liver failure.

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Recombinant C-terminal heparin-binding domain of fibronectin polypeptide protects against liver damage, reduces serum inflammatory cytokines, and decreases mortality in acute liver failure.

This examine aimed to judge the impact of recombinant C-terminal heparin-binding area of fibronectin (FNCHBD) polypeptide on live-damage safety, irritation, and mortality in acute liver failure (ALF) mice. 25 mice have been randomly divided into 5 teams: regular controls, lipopolysaccharide (LPS)/D-galactosamine (GalN), 5 mg/kg FNCHBD, 10 mg/kg FNCHBD and 20 mg/kg FNCHBD teams. Blood samples have been obtained at 9 h after remedy for measurement of liver indexes and inflammatory cytokine ranges, and livers have been acquired for H&E and TUNEL staining assays.

90 mice (18 mice in every group) have been randomly divided into 5 teams for mortality evaluation after LPS/GalN administration at 48 h. In comparison with LPS/GalN group, ranges of blood liver indexes together with AST, ALT and TBIL have been decreased in FNCHBD polypeptide-treated teams. H&E staining disclosed FNCHBD polypeptide protected cell morphology and histomorphology, and necrosis charges in FNCHBD polypeptide-treated teams have been decrease in comparison with LPS/GalN group. TUNEL staining assay revealed cell apoptosis was inhibited in FNCHBD polypeptide-treated teams in comparison with LPS/GalN group.

Serum inflammatory cytokines together with TNF-α, IL-1β, and IL-6 have been lowered in FNCHBD polypeptide-treated teams in comparison with LPS/GalN group. As to mortality charge, it was solely decreased in 10 mg/kg FNCHBD and 20 mg/kg FNCHBD teams however not in 5 mg/kg FNCHBD in comparison with LPS/GaIN group. As well as, most results of FNCHBD offered in a dose-dependent method. FNCHBD polypeptide protects towards liver injury, inhibits elevation of serum inflammatory cytokines, and reduces mortality in ALF.

Cytokines are a household of signaling polypeptides concerned in intercellular interactions within the strategy of the immune response, in addition to within the regulation of quite a few regular physiological capabilities. Cytokines are utilized in medication for the remedy of most cancers, immune issues, viral infections, and different socially vital illnesses, however the extent of their use is restricted by the excessive manufacturing price of the lively agent. The event of this space of pharmacology is related to the success of genetic engineering, which permits the manufacturing of serious quantities of protein by transgenic organisms. The overview discusses the most recent advances within the manufacturing of varied cytokines with using genetically modified crops.

In vitro spleen cell cytokine responses of grownup mice immunized with a recombinant PorA (main outer membrane protein [MOMP]) from Campylobacter jejuni.

There isn’t a info on cytokine profiles to be used as markers of safety in Campylobacter jejuni an infection. To check this, we used outer membrane protein (MOMP [PorA]) because the vaccine for defense and spleen cell cytokines as markers of safety. We cloned and expressed porA from C. jejuni111 and immunized mice by the intraperitoneal route. Subsequently, mice have been orally challenged with stay C. jejuni 111. The vaccine induced safety as evidenced by lowered fecal excretion of C. jejuni111. Cytokines have been measured in vitro after stimulation of spleen cells with MOMP. The degrees of pro-inflammatory cytokines, IL-12, TNF-α, IL-17A and IL-17F have been related in management and take a look at mice.

The degrees of pro-inflammatory cytokines, IL-2 and IFN-γ have been larger in management mice than in take a look at mice, and the degrees of pro-inflammatory cytokines, IL-Eight and IL-1β have been larger in take a look at mice than in management mice. Among the many two anti-inflammatory cytokines, the degrees have been related for IL-10 however larger for IL-Four in take a look at mice than in management mice. Ratios of pro-inflammatory to anti-inflammatory cytokines confirmed a bias in direction of an anti-inflammatory response in favor of antibody manufacturing reflecting the function of antibodies in immunity. Cytokine manufacturing patterns by spleen cells could also be used as markers of safety within the mouse mannequin.

Recombinant C-terminal heparin-binding domain of fibronectin polypeptide protects against liver damage, reduces serum inflammatory cytokines, and decreases mortality in acute liver failure.

Anti-tumoral Results of Recombinant Lactococcus lactis Pressure Secreting IL-17A Cytokine.

Interleukin-17A (IL-17A) is a pro-inflammatory cytokine produced by TH17 cells that participates and contributes in host protection and autoimmune illness. We now have just lately reported antitumor properties of the probiotic pressure of Lactobacillus casei BL23 in mice and TH17 cells was proven to play an essential function on this helpful impact. As a way to higher perceive the function of IL-17A in most cancers, we constructed a recombinant pressure of Lactococcus lactis producing this cytokine and we decided its organic exercise in: (i) a bioassay take a look at for the induction of IL-6 manufacturing by murine fibroblasts 3T3 L1 cells line and (ii) in a mouse allograft mannequin of human papilloma virus (HPV)-induced most cancers.

Our information present that recombinant L. lactis produces and effectively secretes biologically lively IL-17A cytokine. Apparently, ∼26% of mice intranasally handled with L. lactis-IL-17A and challenged with TC-1 cells remained tumor free over the experiment, in distinction to manage mice handled with the wild kind pressure of L. lactis which developed 100% of aggressive tumors. As well as, the median measurement of the ∼74% tumor-bearing mice handled with recombinant L. lactis-IL-17A, was considerably decrease than mice handled with L. lactis-wt. Altogether, our outcomes reveal that intranasal administration with L. lactis secreting IL-17A leads to a partial safety towards TC-1-induced tumors in mice, confirming antitumor results of this cytokine in our most cancers mannequin.