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Recombinant cytokines

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western blot protocol

In vivo antitumor activity of a recombinant IL-7/HGFbeta hybrid cytokine in mice.

May 24, 2021 by Bella Watkins
In vivo antitumor activity of a recombinant IL-7/HGFbeta hybrid cytokine in mice.

The immune cytokine interleukin (IL)-7 and the β-chain of hepatocyte progress issue (HGF) mixture to type a naturally occurring heterodimer that stimulates the expansion of frequent lymphoid progenitors and immature B and T lymphoid cells. We’ve got cloned and expressed the heterodimer as a single-chain hybrid cytokine [recombinant (r) IL-7/HGFβ], which stimulates short-term hematopoietic stem cells in addition to lymphoid precursors. Inasmuch as IL-7 and HGF are recognized to have antitumor and protumor actions, respectively, we decided right here whether or not both of those actions is exhibited by rIL-7/HGFβ. We present that the in vivo administration of rIL-7/HGFβ markedly inhibits the expansion of newly initiated and established tumors and the formation of pulmonary metastases in murine fashions of colon most cancers and melanoma.

The antitumor impact of rIL-7/HGFβ correlated with a marked improve within the variety of tumor-infiltrating CD4(+) and CD8(+) T cells and activated dendritic cells. A serious position for these immune cells in tumor suppression was indicated by the shortcoming of rIL-7/HGFβ to inhibit the expansion of tumor cells in vitro and in congenitally athymic mice. Evaluation of interferon-γ-secreting T cells confirmed that the immune response was tumor particular. Our findings justify additional analysis of rIL-7/HGFβ as a novel experimental most cancers remedy.

The scientific manifestations of human Chagas illness are related to distinct and sophisticated host-parasite interactions that straight contain the host’s immune system. On this examine, we analysed the connection between the manufacturing of intracytoplasmic cytokines after in vitro stimulation with the recombinant antigens CRA (cytoplasmatic repetitive antigen) or FRA (flagellar repetitive antigen) from Trypanosoma cruzi and the continual cardiac or indeterminate scientific types of Chagas illness. The chagasic affected person teams consisted of 39 people, chosen on the Chagas Illness Unit of the Oswaldo Cruz College Hospital, whom introduced both a cardiac type with out cardiac dilatation (CARD 1), cardiac type with cardiac dilatation (CARD 2) or indeterminate type (IND).

Manufacturing of proinflammatory cytokines within the human THP-1 monocyte cell line following induction by Tp0751, a recombinant protein of Treponema pallidum.

The tissue destruction attribute of syphilis an infection could also be brought on by irritation attributable to Treponema pallidum and the following immune responses to the pathogen. T. pallidum membrane proteins are considered potent inducers of irritation in the course of the early levels of an infection. Nonetheless, the precise membrane proteins that induce inflammatory cytokine manufacturing are usually not recognized, nor are the molecular mechanisms liable for triggering and sustaining the inflammatory cascades. Within the current examine, Tp0751 recombinant protein from T. pallidum was discovered to induce the manufacturing of proinflammatory cytokines, together with TNF-alpha, IL-1beta and IL-6, in a THP-1 human monocyte cell line.

The sign transduction pathways concerned within the manufacturing of those cytokines have been then additional investigated. No inhibition of TNF-a, IL-1beta, or IL-6 manufacturing was noticed following remedy with the SAPK/JNK particular inhibitor SP600125 or with an ERK inhibitor PD98059. In contrast, anti-TLR2 mAb, anti-CD14 mAb, and the p38 inhibitor SB203580 considerably inhibited the manufacturing of all three cytokines. As well as, pyrrolidine dithiocarbamate (PDTC), a particular inhibitor of NF-kappaB, profoundly inhibited the manufacturing of those cytokines.

Tp0751 remedy strongly activated NF-kappaB, as revealed by Western blotting. Nonetheless, NF-kappaB translocation was considerably inhibited by remedy with PDTC. These outcomes indicated that TLR2, CD14, MAPKs/p38, and NF-kappaB could be implicated within the inflammatory response brought on by T. pallidum an infection. Blood samples have been obtained from these sufferers and cultured within the presence of CRA or FRA. The cytokines produced by lymphocytes and monocytes after antigen stimulation have been analysed by stream cytometry.

In vivo antitumor activity of a recombinant IL-7/HGFbeta hybrid cytokine in mice.

Impact of recombinant Panton-Valentine leukocidin in vitro on apoptosis and cytokine manufacturing of human alveolar macrophages.

Panton-Valentine leukocidin (PVL) is related to uncommon instances of necrotizing pneumonia that happen in in any other case wholesome people. Human alveolar macrophages (HAMs) are main effector cells in host protection in opposition to infections. Nonetheless, the influence of PVL on HAMs is unsure. We evaluated the position of PVL in cytotoxicity and manufacturing of inflammatory cytokines secreted by HAMs. HAMs have been purified from bronchoalveolar lavage fluid. Recombinant PVL (rPVL) was used within the examine to intervene with HAM apoptosis and cytokine manufacturing in vitro.

Hoechst 33342 fluorescence staining, transmission electron microscopy examination, and stream cytometry indicated that rPVL (10 nmol/L) remedy resulted in HAMs with markedly apoptotic traits, and HAMs handled with rPVL at 100 nmol/L confirmed clear indication of necrosis. A remedy of rPVL at 10 nmol/L elicited the secretion of IL-10 by HAMs relative to untreated management cells, however there was a slight lower within the constitutive secretion of tumor necrosis issue (TNF)-alpha. Our outcomes point out that PVL-treated samples decreased HAM viability, resulting in apoptosis at low concentrations and necrosis at excessive concentrations.

Polystyrene-bottomed 96-well plate (ELISA)

CT361 U-CyTech 6
EUR 73

Black 96 Well Plate, 1EA

X025-1EA Arbor Assays 1EA
EUR 73

Adapter for 96 well plate

3510147 Atto 1unit
EUR 5581

Streptavidin coated 96-well Plate

6523-5 Biovision
EUR 327

96 well filter plate (960ul each well)

SD5006 Bio Basic 12UNIT
EUR 96.54

96 WELL CLEAR PCR PLATE FOR ABI

PCR-96-AB-C CORNING 10/pk
EUR 167
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY

P-DW-12-HC CORNING 5/pk
EUR 152
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

Clear Coated 96 Well Plate, 1EA

C107-1EA Arbor Assays 1EA
EUR 189

96 well filter plate (no filter)

SD5006-EMPTY Bio Basic 12UNIT
EUR 87.41

96 WELL CLEAR PCR HALF SKIRT AMPLIFICATION PLATE

PCR-96-HS-C CORNING 10/pk
EUR 159
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

96 WELL SITTING DROP HIGH THROUGHPUT CRYSTALLOGRAPHY PLATE.

CP-AXYGEM-96-50 CORNING 10/pk
EUR 457
Description: Crystallography Plates; Crystallography Plates - Axygen

96 well DNA plate with membrane (960ul each well)

SD5007 Bio Basic 12UNIT
EUR 242.7

96 well RNA plate with membrane (960ul each well)

SD5009 Bio Basic 12UNIT
EUR 260.97

2 x Clear 96 well Plate, 2EA

X003-2EA Arbor Assays 2EA
EUR 65

5 x Clear 96 well Plate, 5EA

X003-5EA Arbor Assays 5EA
EUR 80

2 x Clear 96 well Plate, 2EA

X018-2EA Arbor Assays 2EA
EUR 65

4 x Clear 96 Well Plate, 4EA

X018-4EA Arbor Assays 4EA
EUR 87

Black Half Area 96 Well Plate, 1EA

X023-1EA Arbor Assays 1EA
EUR 80

96-Well Plate Viral DNA Miniprep Kit

VT92032 Bio Basic 2XPlates, 192prep
EUR 413.22

96-Well Plate PCR Products Purification Kit

BS365 Bio Basic 5XPlates, 480prep
EUR 250.31

96-Well Plate PCR Products Purification Kit

BS3652 Bio Basic 2xPlates, 192prep
EUR 155.16

96-Well Plate DNA Cleanup Miniprep Kit

BS369 Bio Basic 5XPlates, 480prep
EUR 250.31

96-Well Plate DNA Cleanup Miniprep Kit

BS3692 Bio Basic 2xPlates, 192prep
EUR 155.16

96-Well Plate Plasmid DNA Miniprep Kit

BS415 Bio Basic 5XPlates, 480prep
EUR 250.31

96-Well Plate Plasmid DNA Miniprep Kit

BS4152 Bio Basic 2XPlates, 192prep
EUR 155.16

Polystyrene-bottomed 96-well plate + lid (ELISPOT)

CT365 U-CyTech 5
EUR 78

96 Well Breast Tumor Genomic DNA Plate

D8235086-1 Biochain 1 plate
EUR 316

96 Well Colon Tumor Genomic DNA Plate

D8235090-1 Biochain 1 plate
EUR 316

96 Well Lung Tumor Genomic DNA Plate

D8235152-1 Biochain 1 plate
EUR 316

96 Well Stomach Tumor Genomic DNA Plate

D8235248-1 Biochain 1 plate
EUR 316

OxiSelect 96-Well Comet Assay Kit

STA-355 Cell Biolabs 96 assays
EUR 427
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Kit

STA-355-5 Cell Biolabs 5 x 96 assays
EUR 1633
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.

OxiSelect 96-Well Comet Assay Slide

STA-356 Cell Biolabs 1 slide
EUR 183
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

OxiSelect 96-Well Comet Assay Slide

STA-356-5 Cell Biolabs 5 slides
EUR 519
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.

96-Well ROCK Activity Assay Kit

STA-416 Cell Biolabs 96 assays
EUR 856
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

96-Well ROCK Activity Assay Kit

STA-416-5 Cell Biolabs 5 x 96 assays
EUR 3524
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.

Radius 96-Well Cell Migration Assay

CBA-126 Cell Biolabs 96 assays
EUR 572
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

Radius 96-Well Cell Migration Assay

CBA-126-5 Cell Biolabs 5 x 96 assays
EUR 2248
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.

CytoSelect 96-well Cell Transformation Assay

CBA-130 Cell Biolabs 96 assays
EUR 722
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Cell Transformation Assay

CBA-130-5 Cell Biolabs 5 x 96 assays
EUR 2886
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.

CytoSelect 96-well Phagocytosis Assay (Zymosan)

CBA-224 Cell Biolabs 96 assays
EUR 722
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

CytoSelect 96-well Phagocytosis Assay (Zymosan)

CBA-224-5 Cell Biolabs 5 x 96 assays
EUR 2938
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

1.2ML 96 WELL DEEP WELL PLATE HIGH CLARITY PRE-STERILIZED

P-DW-12-HC-S CORNING 5/pk
EUR 207
Description: Deep Well/Assay Plates - Axygen; Deep-well plates - Axygen

SpotLiter? 96 well Plate Light Tracker (foot pedal & USB included)

ACT-SL-96 ACTGene
EUR 647

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1292 Bio Basic 2XPlates, 192prep
EUR 175.71

96-Well Plate Bacteria Genomic DNA Miniprep Kit

SK1295 Bio Basic 5XPlates, 480prep
EUR 324.92

96-well Plasmid DNA Extraction Kit (1 plate)

FAPWE-96001 Favorgen 1 prep
EUR 146

96 Well Plate RNA Cleanup and Concentration Kit

BRC1251 Bio Basic 2xPlates, 192prep
EUR 250.31

96-Well Plate Animal Genomic DNA Miniprep Kit

BS437 Bio Basic 5XPlates, 480prep
EUR 362.98

96-Well Plate Animal Genomic DNA Miniprep Kit

BS4372 Bio Basic 2XPlates, 192prep
EUR 195.5

96 Well Plate Yeast Genomic DNA Miniprep Kit

BS8357 Bio Basic 2XPlates, 192prep
EUR 195.5

96-Well Plate Plant Genomic DNA Miniprep Kit

BS8361 Bio Basic 2XPlates, 192prep
EUR 195.5

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92031 Bio Basic 2Plates, 192prep
EUR 175.71

96 Well Plate Blood Genomic DNA Miniprep Kit

BT92032 Bio Basic 5Plates, 480prep
EUR 324.92

Block for 96 well plate, WSC-2620/30

4002659 Atto 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

PVDF membrane-bottomed 96-well plate + lid (ELISPOT)

CT366 U-CyTech 5
EUR 224

96 Well ExoELISA plate (12x8 well strips, pack of 10 plates)

EXOEL-96P-1 SBI 10 Plates
EUR 213

96-well Checkpoint Kinase Activity Assay Kit

STA-414 Cell Biolabs 96 assays
EUR 757
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

96-well Checkpoint Kinase Activity Assay Kit

STA-414-5 Cell Biolabs 5 x 96 assays
EUR 3060
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.

CytoSelect 96-well Anoikis Assay (Colorimetric/Fluorometric)

CBA-081 Cell Biolabs 96 assays
EUR 606
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.

96-Well Assay Plates, Non-Treated, Clear, Round Bottom 1 Plate/Pack, 100/Unit

25-224 Genesee Scientific 100 plates
EUR 299
Description:

Suitable for cell culture but only for suspension cells

  • Optically clear with low autofluorescence for distortion free microscopy
  • Low evaporation lid reduces evaporation and prevents contamination
  • Exterior stacking ring on lid for added stability when stacking
  • Certified RNase & DNase-Free, Non-Pyrogenic & Non-Cytotoxic
  • Sterilized by Gamma Irradiation to a Sterility Assurance Level of 10^-10

Protein A-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTA55-5P Alpha Diagnostics 1 PK
EUR 263

Protein G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTG65-5P Alpha Diagnostics 1 PK
EUR 263

Mouse anti-Osteopontin Clear Coated 96 Well Plate, 1EA

C075-1EA Arbor Assays 1EA
EUR 178

Mouse anti-Prolactin Clear Coated 96 Well Plate, 1EA

C140-1EA Arbor Assays 1EA
EUR 178

Mouse anti-Myeloperoxidase Clear Coated 96 Well Plate, 1EA

C223-1EA Arbor Assays 1EA
EUR 178

Goat anti-Mouse, Coated White 96 Well Plate, 1EA

X010-1EA Arbor Assays 1EA
EUR 109

Goat anti-Rabbit, Coated White 96 Well Plate, 1EA

X014-1EA Arbor Assays 1EA
EUR 109

Goat anti-Rabbit, Coated Clear 96 Well Plate, 1EA

X016-1EA Arbor Assays 1EA
EUR 109

2 x Black Half Area 96 Well Plate, 2EA

X037-2EA Arbor Assays 2EA
EUR 94

Donkey anti-Sheep, Coated Clear 96 Well Plate, 1EA

X061-1EA Arbor Assays 1EA
EUR 109

Donkey anti-Sheep, Coated White 96 Well Plate, 1EA

X063-1EA Arbor Assays 1EA
EUR 109

96 Well PCR Plates, Fits ABI Fast Plate Machines

GP615 GenDepot 25 plates
EUR 212

COOLSINK XT 96U, 96 WELL U-BOTTOM PLATE MODULE

432071 CORNING 1/pk
EUR 345
Description: BioCision; BioCision CoolBox

SINGLE BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480130 CORNING 1/pk
EUR 223
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL MICROTITER PLATE OR 4 SLIDES

480131 CORNING 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 96 WELL PCR PLATE, SKIRTED OR NONSKIRTED

480132 CORNING 1/pk
EUR 218
Description: Lab Equipment; Constant Temperature Equipment

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS585 Bio Basic 5XPlates, 480prep
EUR 487.82

96 Well Plate Bacterial Total RNA Miniprep Super Kit

BS5852 Bio Basic 2XPlates, 192prep
EUR 250.31

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92028 Bio Basic 2XPlates, 192prep
EUR 212.25

Ultra-Fast 96-Well Plate Plasmid DNA Miniprep Kit

BS92029 Bio Basic 5XPlates, 480prep
EUR 364.5

Adaptor for 96-well PCR plate, accessory for AgileSealer?

APS-200-96PCR ACTGene
EUR 351

Block for 96 deep-well plate, WSC-2620/30

4002661 Atto 1unit
EUR 382
Description: Bl ock for WSC-2620 / 2630

96 WELL BARCODED H1-H12, CLEAR PCR PLATE FOR ABI, 0,2ML

PCR-96-AB-C-PBC CORNING 10/pk
EUR 195
Description: PCR Plates & Tubes; A354 PCR Plate-Axygen

CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay (96 assays)

CBA-320 Cell Biolabs 96 assays
EUR 537
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.

DH10B Chemically Competent Cells (ig10B) - 1x96 well plate - 20µl/well

1018-96 Intact Genomics 1/EA
EUR 518

Clear 384 Well Plate

X105-1EA Arbor Assays 1EA
EUR 112

CytoSelect 96-well Collagen Cell Invasion Assay, Fluorometric

CBA-112-COL Cell Biolabs 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I.

CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric

CBA-112-LN Cell Biolabs 96 assays
EUR 757
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin.

CytoSelect 96-well Phagocytosis Assay (Red Blood Cell)

CBA-220 Cell Biolabs 96 assays
EUR 635
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.

96-well Cellular Senescence Assay (SA ?-Gal Activity)

CBA-231 Cell Biolabs 120 assays
EUR 635
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

96-well Cellular Senescence Assay (SA ?-Gal Activity)

CBA-231-5 Cell Biolabs 5 x 120 assays
EUR 2648
Description: Our Cellular Senescence Activity Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Quantify senescence using a fluorescence plate reader.

Protein A&G-coated ELISA plate (8 well strips, 96 wells/plate) 5 plates/pack

PRTAG75-5P Alpha Diagnostics 1 PK
EUR 286

Goat anti-Mouse, 1 x Whole Well, Clear Coated 96 Well Plate, 1EA

X011-1EA Arbor Assays 1EA
EUR 102

MESG *Phosphate assay reagent*

21600 AAT Bioquest 5 mg
EUR 115

Mouse anti-Cystatin C Clear Coated 96 Well Plate, 1EA

C033-1EA Arbor Assays 1EA
EUR 178
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Sort I interferons (IFNs) are thought-about to be essential mediators of innate immunity attributable to their inherent antiviral exercise, capacity to drive the transcription of quite a few genes concerned in viral clearance, and their position within the initiation of innate and adaptive immune responses. Because of the central position of kind I IFNs, we sought to find out their significance within the era of immunity to a recombinant vaccine vector fowlpox virus (FPV). In analyzing the position of kind I IFNs in immunity to FPV, we present that they’re vital to the secretion of quite a few innate and proinflammatory cytokines, together with kind I IFNs themselves in addition to interleukin-12 (IL-12), tumor necrosis factor-alpha (TNF-alpha), IL-6, and IL-1beta, and that deficiency results in enhanced virus-mediated antigen expression.

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  • Anti-tumoral Effects of Recombinant Lactococcus lactis Strain Secreting IL-17A Cytokine. (7)
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  • In vitro spleen cell cytokine responses of adult mice immunized with a recombinant PorA (major outer membrane protein [MOMP]) from Campylobacter jejuni. (6)
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Recent Posts

  • DNA Replication Fork
  • Genetic Variation in Meiosis
  • Protein Synthesis
  • In vivo antitumor activity of a recombinant IL-7/HGFbeta hybrid cytokine in mice.
  • The local cytokine and growth factor response to recombinant human bone morphogenetic protein-2 (rhBMP-2) after spinal fusion.

Tags

particles particles pcr pcram pcrb pcr covid test pcr covid testing pcrdfans pcre pcr kits price pcr test for coronavirus pcr test for covid 19 pcr testing for covid-19 pcr tests pcr unit peptides for nerve repair peptides for sale peptides for skin peptides for weight loss peptides international peptides rodan and fields peptides serum peptides supplement peptides t3 peptides tadalafil serums.com serums for hair growth serums for men serums for sensitive skin serums for wrinkles serums in anoka serums in anoka mn serums mn serums osrs serums peru serumstat serums ulta serums vs creams serums with glycerin serums with spf SOCS1 SOCS2 SOCS3 SOCS4 SOCS5 SOCS6
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