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Targeting a cysteine protease from a pathobiont alleviates experimental arthritis.

Several lines of evidence suggest that the pathobiont Porphyromonas gingivalis is involved in the development and/or progression of auto-inflammatory diseases. This bacterium produces cysteine proteases, such as gingipain RgpA, endowed with the potential to induce significant bone loss in model systems and in patients.

We sought to gain further insight into the role of this pathobiont in rheumatoid arthritis (RA) and to identify novel therapeutic targets for auto-inflammatory diseases.We profiled the antibody response to RgPA-specific domains in patient sera.

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Description: Cell proliferation and toxicity test

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Description: Cell-proliferation-and-toxicity-test

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Description: 509-80-8

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Description: Chromatography Reagents

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Description: Chromatography Reagents

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Description: Chromatography Reagents

Custom production of antibodies in 1 Goat using supplied antigen (std 63 days protocol)

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Custom production of antibodies in 5 Mice using supplied antigen (std 63 days protocol)

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Custom production of antibodies in 1 Sheep using supplied antigen (std 63 days protocol)

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Custom production of antibodies in 1 Rabbit using supplied antigen (std 63 days protocol)

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CHI-2 Alpha Diagnostics 1 1365.6 EUR

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Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)

RAT-5 Alpha Diagnostics 1 1365.6 EUR

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Thymol Blue

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We also tested the potential protective effects of RgpA domains in an experimental arthritis model.Pre-immunization of rats with purified recombinant RgpA domains alleviated arthritis in the joints of the rodents and reduced bone erosion.

Using a functional genomics approach at both the mRNA and protein levels, we report that the pre-immunizations reduced arthritis severity by impacting a matrix metalloprotease characteristic of articular injury, a chemokine known to be involved in recruiting inflammatory cells, and three inflammatory cytokines.

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