Efficient mucosal vaccination of a novel classical swine fever virus E2-Fc fusion protein mediated by neonatal Fc receptor.

Classical swine fever (CSF) remains one of the most important highly contagious and fatal viral disease of swine with high morbidity and mortality. CSF is caused by classical swine fever virus (CSFV), a small, enveloped RNA virus of the genus Pestivirus.

The aim of this study was to construct the a novel CSFV Fc-fusion recombinant protein and evaluate the efficacy as a vaccine against CSFV. Here, we obtained a novel subunit vaccine expressing CSFV E2 recombinant fusion protein in CHO-S cells.

Alamar Blue Cell Viability Assay Kit
E37F026-100tests 100 tests
EUR 60
Description: Cell proliferation and toxicity test

Alamar Blue Cell Viability Assay Kit
E37F026-3000tests 3000 tests
EUR 600
Description: Cell proliferation and toxicity test

Alamar Blue Cell Viability Assay Kit
E37F026-500tests 500 tests
EUR 191.67
Description: Cell proliferation and toxicity test

Alamar Blue Cell Viability Assay Kit
MBS8579843-1000Tests 1000Tests
EUR 330

Alamar Blue Cell Viability Assay Kit
MBS8579843-100Tests 100Tests
EUR 170

Alamar Blue Cell Viability Assay Kit
MBS8579843-3000Tests 3000Tests
EUR 555

Alamar Blue Cell Viability Assay Kit
MBS8579843-500Tests 500Tests
EUR 255

Alamar Blue Cell Viability Assay Kit
MBS8579843-5x100Tests 5x100Tests
EUR 720

Alamar Blue Cell Viability Assay Reagent
A7631
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  • 1 mL - 100 Tests
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Alamar Blue
MBS638941-25mL 25mL
EUR 650

Alamar Blue
MBS638941-5x25mL 5x25mL
EUR 2765

Alamar-Blue-Cell-Viability-Assay-Kit
E37F026 1000-tests
EUR 300
Description: Cell-proliferation-and-toxicity-test

AlamarBlue Cell Viability Assay Reagent
AR4002 10ml
EUR 90

AlamarBlue Cell Viability Assay Reagent
AR4002-10ml 10ml
EUR 184.8

AlamarBlue Cell Viability Assay Reagent
AR4002-25ml 25ml
EUR 338.4

Mitraphyllin - exact weight protocol
M374000 250mg
EUR 1200
Description: 509-80-8

Mitraphyllin - exact weight protocol
TRC-M374000-250MG 250 mg Ask for price

Cyflufenamid-d5 - Exact Weight Protocol
C989602 25mg
EUR 12800

Cyflufenamid-d5 - Exact Weight Protocol
TRC-C989602-25MG 25 mg Ask for price

Antibody column package, 1-step protocol
An-305P 1 kit
EUR 560
Description: Chromatography Reagents

Antibody column package, 2-step protocol
An-306P 1 kit
EUR 2032
Description: Chromatography Reagents

Thiol Modified Oligo Disulfide Reduction Protocol
PT_THIOL 1 Each Ask for price

His-tagged column package, 2-step protocol
His-108P 1 kit
EUR 1832
Description: Chromatography Reagents

Trypan Blue Assay Kit
AR1175 1kit (for 1000 assays)
EUR 78

Zymo-Spin™ ChIP Kit (10 preps) Mechanical Shearing Protocol
D5209 10 Preparations Ask for price

Zymo-Spin™ ChIP Kit (25 preps) Mechanical Shearing Protocol
D5210 25 Preparations Ask for price

Trypan Blue Cell Viability Assay
TBS2008 100 mL
EUR 70

Antibody column package, modern Protein A (1-step purification protocol)
An-002P 1 kit
EUR 560
Description: Chromatography Reagents

Antibody column package, modern Protein A (2-step purification protocol)
An-003P 1 kit
EUR 2032
Description: Chromatography Reagents

Trypan Blue Staining Cell Assay Kit
APB0003-10 5ml
EUR 0.1

Magnesium assay kit (Xyridil Blue-I)
CMG-035 100 tests
EUR 197.29
Description: Traceelementsassay

Cell Cycle Assay Kit (Blue Fluorescence)
E-CK-A353-100Assays 100Assays
EUR 128
Description: Cell Cycle

Cell Cycle Assay Kit (Blue Fluorescence)
E-CK-A353-20Assays 20Assays
EUR 45
Description: Cell Cycle

Cell Cycle Assay Kit (Blue Fluorescence)
E-CK-A353-50Assays 50Assays
EUR 78
Description: Cell Cycle

Cell Cycle Assay Kit (Blue Fluorescence)
E-CK-A353-each each Ask for price
Description: Cell Cycle

Glucose Uptake Capacity Assay Kit (Blue)
G9820 1 Kit Ask for price

Custom production of antibodies in 1 Goat using supplied antigen (std 63 days protocol)
GOT-1 1
EUR 2252.4

Custom production of antibodies in 5 Mice using supplied antigen (std 63 days protocol)
MIC-5 1
EUR 1365.6

Custom production of antibodies in 1 Sheep using supplied antigen (std 63 days protocol)
SHP-1 1
EUR 2252.4

Custom production of antibodies in 1 Rabbit using supplied antigen (std 63 days protocol)
RAB-1 1
EUR 790.8

Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*
13803-200Tests 200 Tests
EUR 334
Description: Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

Custom production of antibodies in 2 Chickens using supplied antigen (std 63 days protocol)
CHI-2 1
EUR 1365.6

Custom production of antibodies in 2 Rabbits using supplied antigen (std 63 days protocol)
RAB-2 1
EUR 1365.6

Custom production of antibodies in 2 G. Pigs using supplied antigen (std 63 days protocol)
GPI-2 1
EUR 1365.6

Cell Meterâ„¢ Autophagy Assay Kit *Blue Fluorescence*
23000-200Tests 200 Tests
EUR 222
Description: Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway.

cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251 - EACH
GERPN2251 EACH
EUR 770.85

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)
RAT-5 1
EUR 1365.6

PhosphoWorksâ„¢ Colorimetric Phosphate Assay Kit *Blue Color*
21665-1000Tests 1000 Tests
EUR 222
Description: Phosphate is involved in many biological reactions.

Cell Meterâ„¢ Cell Viability Assay Kit *Blue Fluorescence*
22785-500Tests 500 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Amplite® Fluorimetric NAD Assay Kit *Blue Fluorescence*
15280 200 Tests
EUR 398
Description: Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.

Amplite® Fluorimetric NAD Assay Kit *Blue Fluorescence*
15280-200Tests 200 Tests
EUR 390
Description: Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.

Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*
15281 200 Tests
EUR 398
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.

Amplite® Fluorimetric NADP Assay Kit *Blue Fluorescence*
15281-200Tests 200 Tests
EUR 390
Description: Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors for many enzyme reactions found in living cells.

PhosphoWorksâ„¢ Fluorimetric Pyrophosphate Assay Kit *Blue Fluorescence*
21611-200Tests 200 Tests
EUR 222
Description: Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.

Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*
13620-200Tests 200 Tests
EUR 222
Description: Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.

Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
12602-200Tests 200 Tests
EUR 334
Description: Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.

Amplite® Colorimetric Peroxidase (HRP) Assay Kit *Blue Color*
11551-500Tests 500 Tests
EUR 222
Description: Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*
13502-500Tests 500 Tests
EUR 334
Description: Caspases play important roles in apoptosis and cell signaling.

Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*
11952-500Tests 500 Tests
EUR 222
Description: Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

Total protein quantitative assay kit (Coomassie brilliant blue method, with standard)
MBS2540545-100Assays 100Assays
EUR 220

Total protein quantitative assay kit (Coomassie brilliant blue method, with standard)
MBS2540545-100Tests 100Tests
EUR 220

Total protein quantitative assay kit (Coomassie brilliant blue method, with standard)
MBS2540545-500Assays 500Assays
EUR 285

Total protein quantitative assay kit (Coomassie brilliant blue method, with standard)
MBS2540545-500Tests 500Tests
EUR 285

Total protein quantitative assay kit (Coomassie brilliant blue method, with standard)
MBS2540545-5x100Tests 5x100Tests
EUR 1300

Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
11560 200 Tests
EUR 341
Description: Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.

Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
11560-200Tests 200 Tests
EUR 334
Description: Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.

Cell Meterâ„¢ Caspase 8 Activity Apoptosis Assay Kit *Blue Fluorescence*
22812-200tests 200 tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

Cell Meterâ„¢ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*
22813-200Tests 200 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

Live or Deadâ„¢ Cell Viability Assay Kit *Red/Blue Dual Fluorescence*
22788-200Tests 200 Tests
EUR 334
Description: This Live or Deadâ„¢ Cell Viability Assay Kit uses two fluorescent indicators: Cellbriteâ„¢ Red (Ex/Em = 613/631 nm) for labeling viable cells and a cell-impermeable DNA-binding dye Nuclear Blueâ„¢ DCS1 (Ex/Em = 360/450 nm) for labeling dead cells with damaged membranes.

alamarBlue
MBS238967-25mL 25mL
EUR 300

alamarBlue
MBS238967-5x25mL 5x25mL
EUR 1170

Cell Meterâ„¢ Caspase 3/7 Activity Apoptosis Assay Kit *Blue Fluorescence*
22795-200Tests 200 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Bucculiteâ„¢ Flow Cytometric XdU Cell Proliferation Assay Kit *Blue Laser-Comptatible*
22323-100Tests 100 Tests
EUR 516
Description: Monitoring cell proliferation is one of the most reliable methods to assess cell viability, cell cycles and genotoxicity.

Cell Meterâ„¢ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*
11504-100Tests 100 Tests
EUR 278
Description: Hydrogen peroxide is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

Cell Meterâ„¢ Cell Viability Assay Kit *Blue Fluorescence with 405 nm Excitation*
22784-500Tests 500 Tests
EUR 222
Description: There are a variety of parameters that can be used to monitor cell viability.

Cell Meterâ„¢ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Blue Fluorescence*
22857-25Tests 25 Tests
EUR 425
Description: Cell Meterâ„¢ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation.

Cell Meterâ„¢ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
22835-100Tests 100 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
22828-100Tests 100 Tests
EUR 222
Description: Our Cell Meterâ„¢ assay kits are a set of tools for monitoring cell viability.

Cell Meter™ No-Wash Live Cell Caspase 3/7 Activity Assay Kit *Blue Fluorescence*
20250 200 Tests
EUR 60

OOSA11224-25ML - alamarBlue Accessory Reagent
OOSA11224-25ML 25ml
EUR 279

Cell Meterâ„¢ Phosphatidylserine Apoptosis Assay Kit *Blue Fluorescence Optimized for Microplate Readers*
22790-100Tests 100 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

OOSA11225-100ML - alamarBlue Accessory Reagent
OOSA11225-100ML 100ml
EUR 659

CellCount-Blue Cell Proliferation Assay Kit
C0100-025 250 Assays
EUR 133.2

CellCount-Blue Cell Proliferation Assay Kit
C0100-050 500 Assays
EUR 160.8

CellCount-Blue Cell Proliferation Assay Kit
C0100-100 1000 Assays
EUR 225.6

CellCount-Blue Cell Proliferation Assay Kit
C0100-500 5000 Assays
EUR 639.6

CellQuanti-Blue Cell Viability Assay Kits
CQBL-05K 5000
EUR 219

CellQuanti-Blue Cell Viability Assay Kits
CQBL-10K 10000
EUR 349

Protocadherin 15 (PCDH15) Magnetic Luminex Assay Kit
LKU600367-96T 96T
EUR 993.6

Protocadherin 15 (PCDH15) Magnetic Luminex Assay Kit
LKU600378-96T 96T
EUR 966

Protocadherin 20 (PCDH20) Magnetic Luminex Assay Kit
LKU600386-96T 96T
EUR 966

Cell Meterâ„¢ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*
11505-100Tests 100 Tests
EUR 278
Description: Hydrogen peroxide is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

Protocadherin Beta 15 (PCDHb15) Magnetic Luminex Assay Kit
LKU600391-96T 96T
EUR 966

Protocadherin Beta 16 (PCDHb16) Magnetic Luminex Assay Kit
LKU600394-96T 96T
EUR 966

Protocadherin Gamma A2 (PCDHgA2) Magnetic Luminex Assay Kit
LKU600398-96T 96T
EUR 966

Protocadherin-11 Antibody
C11648-100ul 100μl
EUR 217
Description: Protocadherin-11 Rabbit Polyclonal Antibody

Protocadherin-11 Antibody
C11648-50ul 50μl
EUR 143.5
Description: Protocadherin-11 Rabbit Polyclonal Antibody

1L ProtoStain Blue - 1L
NAT1112 1L
EUR 174.79

Alanine Assay Kit
GWB-AXR311 100 assays Ask for price

Alanine Assay Kit
MBS169555-200Assays 200Assays
EUR 615

Alanine Assay Kit
MBS169555-5x200Assays 5x200Assays
EUR 2820

Alanine Assay Kit
MET-5093 200 assays
EUR 368

Alanine Assay Kit
55R-1483 100 assays Ask for price

Alachlor Assay Kit
AB500071-1 1kit
EUR 670.68

Alachlor Plate Assay Kit
AB500076-1 1kit
EUR 670.68

Alanine Transaminase Assay Kit
MBS822345-100Assays 100Assays
EUR 350

Alanine Transaminase Assay Kit
MBS822345-5x100Assays 5x100Assays
EUR 1510

Alanine aminotransferase Assay Kit
BC008-96T 96T
EUR 110

Alanine aminotransferase Assay Kit
SH0261 60ml×230ml×1
EUR 160

Alanine aminotransferase Assay Kit
SH0282 100T/50Sample
EUR 150

Alanine aminotransferase Assay Kit
SH0388 96T
EUR 168

Sudan Blue II;Oil Blue 35
S688913 1g
EUR 92
Description: 17354-14-2

Sudan Blue II;Oil Blue 35
TRC-S688913-500MG 500 mg Ask for price

Sudan Blue II;Oil Blue 35
TRC-S688913-5G 5 g Ask for price

Sudan Blue II;Oil Blue 35
TRC-S688913-1G 1 g Ask for price

Protein Assay Kit
abx295099-100g 100 µg Ask for price

Protein Assay Kit
abx295099-20g 20 µg
EUR 187.5

Protein Assay Kit
abx295099-50g 50 µg Ask for price

Protein Assay Kit
abx295100-100g 100 µg Ask for price

Protein Assay Kit
abx295100-20g 20 µg
EUR 187.5

Protein Assay Kit
abx295100-50g 50 µg Ask for price

Protein Assay Kit
TBS2005 1000 tests
EUR 178

ECOS Blue (XL1-Blue
FYE107-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE107-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE108-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE108-80VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE109-10VL 100 µl x 10 vials Ask for price

ECOS Blue (XL1-Blue
FYE109-80VL 100 µl x 10 vials Ask for price

Protease Assay Kit
18-439 50 Assays/Unit
EUR 294.42
Description: Assay of General Proteases

Protease Assay Kit
55R-1528 100 assays Ask for price

Alanine Transaminase (ALT) Assay Kit
abx295024-100g 100 µg Ask for price

Alanine Transaminase (ALT) Assay Kit
abx295024-20g 20 µg
EUR 437.5

Alanine Transaminase (ALT) Assay Kit
abx295024-50g 50 µg Ask for price

Alanine Transaminase (ALT) Assay Kit
abx098404-Hitachi7020R150ml4R250ml1 Hitachi 7020; R1: 50ml×4 R2: 50ml×1
EUR 247.2

Alanine Transaminase (ALT) Assay Kit
abx098404-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 247.2

Alanine Transaminase (ALT) Assay Kit
abx098404-Hitachi7170R132ml4R28ml4 Hitachi 7170; R1: 32ml×4 R2: 8ml×4
EUR 247.2

Alanine Transaminase (ALT) Assay Kit
abx098404-Toshiba120R150ml4R250ml1 Toshiba 120; R1: 50ml×4 R2: 50ml×1
EUR 247.2

Alanine Transaminase (ALT) Assay Kit
abx098404-UniversalR160ml2R230ml1 Universal; R1: 60ml×2 R2: 30ml×1
EUR 247.2

Alanine Transaminase (ALT) Assay Kit
abx096001-100Assays 100 Assays
EUR 416.4

Alanine Transaminase (ALT) Assay Kit
abx096001-100l 100 µl
EUR 250

Alanine Transaminase (ALT) Assay Kit
abx096001-1ml 1 ml Ask for price

Alanine Transaminase (ALT) Assay Kit
abx096001-200l 200 µl Ask for price

Proteasome Assay Kit
55R-1341 100 assays Ask for price

Aniline Blue (water soluble) (Methyl Blue)
83344 25 Gms
EUR 4.79
Description: Part A

Creatinine Assay Kit (urine normalizing assay)
MBS480387-1Kit 1Kit
EUR 220

Creatinine Assay Kit (urine normalizing assay)
MBS480387-5x1Kit 5x1Kit
EUR 960

Alanine Aminotraasferase(ALT) assay kit
MBS2540581-48Test 48Test
EUR 230

Alanine Aminotraasferase(ALT) assay kit
MBS2540581-48Tests 48Tests
EUR 230

Alanine Aminotraasferase(ALT) assay kit
MBS2540581-500Tests 500Tests
EUR 500

Alanine Aminotraasferase(ALT) assay kit
MBS2540581-96Test 96Test
EUR 270

Alanine Aminotraasferase(ALT) assay kit
MBS2540581-96Tests 96Tests
EUR 270

Lactic Acid Assay, Colorimetric/Fluorometric Assay
TBS2071-100 100 tests
EUR 320

EnzyChrom Alanine Transaminase Assay Kit
EALT-100 100
EUR 369

Luxol Fast Blue ARN (Solvent Blue 37)
L05250 25G
EUR 324.76
Description: CAS N° 12226-74-3

Alanine Transaminase Microplate Assay Kit
DLSM0002 100 Assays
EUR 210
Description: Detection and Quantification of Alanine Transaminase Activity.

Alanine Transaminase Microplate Assay Kit
ASK1002 100 Assays
EUR 228
Description: Detection and Quantification of Alanine Transaminase Activity.

Alanine Transaminase Microplate Assay Kit
RDSM002 100 Assays
EUR 210
Description: Detection and Quantification of Alanine Transaminase Activity.

Jun B Proto Oncogene (JUNB) Magnetic Luminex Assay Kit
LKU606752-96T 96T
EUR 993.6

Protoporphyrinogen Oxidase (PPOX) Magnetic Luminex Assay Kit
LKU600361-96T 96T
EUR 966

Protoporphyrinogen Oxidase (PPOX) Magnetic Luminex Assay Kit
LKU600364-96T 96T
EUR 1048.8

Navy Blue 018112
N926250 50mg
EUR 7600
Description: 118685-33-9

Navy Blue 018112
TRC-N926250-50MG 50 mg Ask for price

Protein Assay BCA Kit
06385-00 1KIT
EUR 105

PDQ Protease Assay™
0201 Kit
EUR 133.4

BCA Protein Assay Kit
20-abx293001
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  • 100 tests
  • 500 tests
  • 2500 tests

BCA Protein Assay Kit
AR0146 1kit
EUR 121.2

BCA Protein Assay Kit
AR0146-1L 1kit
EUR 547.2

BCA Protein Assay Kit
AR0146-500 1kit
EUR 339.6

BCA Protein Assay Kit
abx090640-100l 100 µl
EUR 137.5

BCA Protein Assay Kit
abx090640-1ml 1 ml Ask for price

BCA Protein Assay Kit
abx090640-200l 200 µl
EUR 175

BCA Protein Assay Kit
20-abx090640
  • Ask for price
  • Ask for price
  • 200 tests
  • 500 tests

BCA Protein Assay Kit
abx098126-100ml 100 ml
EUR 303.6

BCA Protein Assay Kit
EZPQ01-2500T 2500T
EUR 144

BCA Protein Assay Kit
EZPQ01-250T 250T
EUR 20.4

BCA Protein Assay Kit
EZPQ01-500T 500T
EUR 34.8

BCA Protein Assay Kit
K4101-1000 1000T
EUR 80
Description: Protein & Immunology|Protein quantification kits

Functional analysis revealed that CSFV Fc-fusion recombinant protein (CSFV-E2-Fc) could bind to FcγRI on antigen-presenting cells (APCs) and significantly increase IgA levels in serum and feces, inducing stronger mucosal immune response in swine.